They are five-year-olds Tyrique Arthurs and Talrique Arthurs, both of Exchange, Ocho Rios, in the parish.
Both boys are of slim build and about 107 centimetres (3 feet 6 inches) tall, however Talrique is of dark complexion while Tyrique is of brown complexion.
According to reports, the brothers were last seen about 12:26 am at the home of a relative on Jamaica Crescent, in Cornwall Court, St James and have not been heard from since.
When last seen Talrique was dressed in a blue shirt and multi-coloured boxers and Tyrique was wearing a green shirt and blue boxers with green stripes.
Anyone knowing the whereabouts of Tyrique Arthurs and Talrique Arthurs is being asked to contact the Ocho Rios Police at 974-2533, the police’s 119 emergency number or the nearest police station.